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Image Search Results
Journal: Frontiers in Endocrinology
Article Title: Inadequate detection of the FSHR complicates future research on extragonadal FSHR localization
doi: 10.3389/fendo.2023.1095031
Figure Lengend Snippet: Immunohistochemical analysis of FFPE human premenopausal ovary, testis and skin tissue ( A–C , respectively). Polyclonal anti-FSHR antibody was ommitted (A1, B1 and C1) or the sections were incubated with the antibody (A2-3, B2-4, C2-3 in 1:500 dilution). Sections that were not incubated with the anti-FSHR antibody only showed blue nuclear staining. The asterisks show FSHR positive (red/brown) granulosa cells (A3) and possibly FSHR positive Sertoli cells among other positive cells in the seminiferous tubules of the testis (B3, B4). Staining inhibin in ovary tissue reveals how specific granulosa staining can be (A4). Staining of skin tissue with anti-cytokeratin 17 antibody shows specific staining of a hair follicle with its sebaceous gland (C4), as opposed to the aspecific staining throughout the skin tissue section by the anti-FSHR antibody. Magnification: Panels A1-2, B1-2, and C1-2 12.5x; Panels A3-4, B3-4, and C3-4 200x; Panel C4 100x.
Article Snippet: The sections were incubated with either the primary mouse monoclonal antibody directed against the human FSHR (clone FSHR/1400, NSJ Bioreagents; unclear which part of the FSHR it recognizes) or the primary
Techniques: Immunohistochemical staining, Incubation, Staining
Journal: Frontiers in Endocrinology
Article Title: Inadequate detection of the FSHR complicates future research on extragonadal FSHR localization
doi: 10.3389/fendo.2023.1095031
Figure Lengend Snippet: Crystallographic image of the extracellular domain of the FSHR (blue, beige, yellow) folding over its ligand FSH (orange), adapted from Jiang et al. . In yellow the beta strands/leucine-rich repeat domains in amino acid stretch C18-N187 are highlighted, against which the polyclonal anti-FSHR antibody MBS178821 is raised. The blue ribbon represent the amino acids that exon 9 codes for, which lacks in the short FSHR variant.
Article Snippet: The sections were incubated with either the primary mouse monoclonal antibody directed against the human FSHR (clone FSHR/1400, NSJ Bioreagents; unclear which part of the FSHR it recognizes) or the primary
Techniques: Variant Assay